Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1165A
View all KIR2DS5/CD158g Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human KIR2DS5/CD158g
His22-His245
Accession # Q14953

Specificity

Detects human KIR2DS5/CD158g in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR2DS5/CD158g Antibody

Detection of KIR2DS5/CD158g antibody in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g antibody by Flow Cytometry.

Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry.

BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g was stained with Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, filled histogram) or Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Detection of KIR2DS5/CD158g antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DS5/CD158g in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human KIR2DS5/CD158g Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g and Human Peripheral Blood Mononuclear Cells (PBMCs)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DS5/CD158g

Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is an activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unknown.  

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5

Alternate Names

CD158g, NKAT9

Entrez Gene IDs

3810 (Human)

Gene Symbol

KIR2DS5

UniProt

Q14953

Additional KIR2DS5/CD158g Products

Product Documents for Human KIR2DS5/CD158g Antibody

Certificate of Analysis

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Product Specific Notices for Human KIR2DS5/CD158g Antibody

For research use only

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