Human KIR2DS5/CD158g Antibody Summary
Accession # Q14953
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g was stained with Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, filled histogram) or Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Detection of KIR2DS5/CD158g in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human NCAM‑1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is an activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unknown.
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