Human KIR2DS5/CD158g Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8920
MAB8920-SP
Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry.
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Human KIR2DS5/CD158g Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DS5/CD158g in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1165A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human KIR2DS5/CD158g
His22-His245
Accession # Q14953
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry. View Larger

Detection of KIR2DS5/CD158g in BaF3 Mouse Cell Line Transfected with Human KIR2DS5/CD158g by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human KIR2DS5/CD158g was stained with Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, filled histogram) or Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Flow Cytometry Detection of KIR2DS5/CD158g in Human PBMCs by Flow Cytometry. View Larger

Detection of KIR2DS5/CD158g in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human NCAM‑1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: KIR2DS5/CD158g

Killer-immunoglobulin-like receptors (KIR) are polymorphous activating and inhibitory receptors expressed on the surface of NK cells and some T cells. KIR genes are highly homologous. KIR proteins expressing the long (L) cytoplasmic domain are inhibitory, while KIRs with short (S) cytoplasmic domains are activating. Thus, KIR2DS5 is an activating receptor for NK cells, and is thought to play a role in NK cell function in response to a number of conditions. While many KIR proteins bind HLA-class I molecules, the ligand(s) for KIR2DS5 remain unknown.  

Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 5
Entrez Gene IDs
3810 (Human)
Alternate Names
killer cell immunoglobulin-like receptor 2DS5; CD158g; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; KIR2DS5; NKAT9

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