Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all LAIR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR2
Gln22-Pro152
Accession # Q6ISS4

Specificity

Detects human LAIR2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human LAIR1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human LAIR2 Biotinylated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human LAIR2 (Catalog # 2665-LR)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR2

LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1‑3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C‑terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997)  Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

Q6ISS4

Additional LAIR2 Products

Product Documents for Human LAIR2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LAIR2 Biotinylated Antibody

For research use only

Citations for Human LAIR2 Biotinylated Antibody

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