LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1‑3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C‑terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAIR2
Gln22-Pro152
Accession # Q6ISS4
Gln22-Pro152
Accession # Q6ISS4
Specificity
Detects human LAIR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human LAIR1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human LAIR2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human LAIR2 (Catalog # 2665-LR)
Sample: Recombinant Human LAIR2 (Catalog # 2665-LR)
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LAIR2
References
- Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
- Meyaard, L. et al. (1999) J. Immunol. 162:5800.
- Meyaard, L. et al. (1997) Immunity 7:283.
- Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
Long Name
Leukocyte-associated Ig-like Receptor 2
Alternate Names
CD306
Gene Symbol
LAIR2
UniProt
Additional LAIR2 Products
Product Documents for Human LAIR2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LAIR2 Antibody
For research use only
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Protocols
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