Human LAMP‑2/CD107b Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # AF6228AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG
View all LAMP-2/CD107b Primary Antibodies »

Product Specifications

Specificity

Detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human LAMP‑2/CD107b Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LAMP-2/CD107b

Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).

References

  1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
  2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
  3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
  4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
  5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
  6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
  7. Tanaka, Y. et al. (1990) Nature 406:902.
  8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
  9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
  10. Grutzkau, A. et al. (2004) Cytometry 61:62.
  11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
  12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
  13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
  14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
  15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.

Long Name

Lysosome-associated Membrane Glycoprotein 2

Alternate Names

CD107b, LAMP2, LAMPB, LGP110

Entrez Gene IDs

3920 (Human); 16784 (Mouse)

Gene Symbol

LAMP2

UniProt

P13473

Additional LAMP-2/CD107b Products

Product Documents for Human LAMP‑2/CD107b Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LAMP‑2/CD107b Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways
Dendritic Cell Lineage Development Pathway Thumbnail