Human LAMP-2/CD107b Antibody

Catalog # Availability Size / Price Qty
AF6228
AF6228-SP
Detection of Human LAMP2/CD107b by Western Blot.
4 Images
Product Details
FAQs
Supplemental Products
Reviews

Human LAMP-2/CD107b Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP2/CD107b
Leu29-Phe375
Accession # P13473
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Knockout Validated
LAMP‑2/CD107b is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in LAMP‑2/CD107b knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human LAMP2/CD107b antibody by Western Blot. View Larger

Detection of Human LAMP2/CD107b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line, JEG-3 human epithelial choriocarcinoma cell line, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for LAMP2/CD107b at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry LAMP2/CD107b antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

LAMP2/CD107b in HeLa Human Cell Line. LAMP2/CD107b was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6228) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of LAMP-2A antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LAMP-2A in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human LAMP2/CD107b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6228, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Knockout Validated Western Blot Shows Human LAMP-2/CD107b Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human LAMP‑2/CD107b Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LAMP-2/CD107b knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LAMP-2/CD107b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-2/CD107b at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-2/CD107b

Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).

References
  1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
  2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
  3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
  4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
  5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
  6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
  7. Tanaka, Y. et al. (1990) Nature 406:902.
  8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
  9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
  10. Grutzkau, A. et al. (2004) Cytometry 61:62.
  11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
  12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
  13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
  14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
  15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.
Long Name
Lysosome-associated Membrane Glycoprotein 2
Entrez Gene IDs
3920 (Human); 16784 (Mouse)
Alternate Names
CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMP2A; LAMP-2A; LAMPB; LGP110; LGP-96; Lysosomal Associated Membrane Protein 2 ; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human LAMP-2/CD107b Antibody

There are currently no reviews for this product. Be the first to review Human LAMP-2/CD107b Antibody and earn rewards!

Have you used Human LAMP-2/CD107b Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review