Human LC3B Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9390T-100
Detection of LC3B in HeLa Human Cell Line by Flow Cytometry.
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Human LC3B Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LC3B in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1251A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human LC3B synthetic peptide
Accession # Q9GZQ8
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of LC3B antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LC3B in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line either treated with 50 µM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Human LC3B Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC9390T). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LC3B

Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy. 

References
  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. He, H. et al. (2003) J. Biol. Chem. 278:29278.
  3. Kuznetsov, S.A. & V.I. Gelfand (1987) FEBS Let. 212:145.
  4. Weidberg, H. et al. (2011) Ann Rev. Biochem. 80:125.
  5. Weidberg, H. et al. (2010) EMBO J. 29:1792. 
 
Long Name
Microtubule-associated Protein 1 Light Chain 3 beta
Entrez Gene IDs
81631 (Human); 67443 (Mouse); 64862 (Rat)
Alternate Names
Apg8b; ATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; lc3b autophagy marker; LC3B; lc3-i, ii; LC3II; MAP1 light chain 3-like protein 2; MAP1A/1BLC3; MAP1A/MAP1B LC3 B; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 3 b; microtubule associated protein 3 beta; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3B

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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