Human LIFR alpha Alexa Fluor® 700-conjugated Antibody

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FAB249N-100UG
R&D Systems Antibodies
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Human LIFR alpha Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIF R alpha in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 32953
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LIF R alpha
Gln45-Ser833
Accession # P42702
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIFR alpha

The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha  subunit (LIF R alpha, also known as LIF R and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

References
  1. Bazan, J.F. 1990, Proc. Natl. Acad. Sci. USA 87:6934.
  2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
  3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.
Long Name
Leukemia Inhibitory Factor Receptor alpha
Entrez Gene IDs
3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)
Alternate Names
CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR alpha; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS

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