Human LILRA1/LILRB1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB30851A
Detection of LILRA1/LILRB1 in Human Blood Monocytes by Flow Cytometry.
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Human LILRA1/LILRB1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRA1 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) LILRB1 is observed and no cross-reactivity rhILT3, 4, 5, 6, rhLIR-7 or -8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 586326
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRA1
Pro17-Asn461
Accession # O75019
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LILRA1/LILRB1 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of LILRA1/LILRB1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA1/LILRB1 APC‑conjugated Monoclonal Antibody (Catalog # FAB30851A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA1/LILRB1

Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged amino acid (aa) within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.

Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1
Alternate Names
LILRA1/LILRB1

Product Datasheets

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Human LILRA1/LILRB1 APC-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human