Human LILRA5/CD85f Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6754T-025
Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry.
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Human LILRA5/CD85f Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.
Source
Monoclonal Mouse IgG2A Clone # 711828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ILT11/LILRA5
Gly42-Arg268
Accession # A6NI73
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry. View Larger

Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human LILRA5/CD85f Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB6754T) or (B) Mouse IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA5/CD85f

LILRA5, also known as Leukocyte Immunoglobulin-like Receptor 9 (LIR-9), CD85f, and ILT11, is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors.  It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta.  Mature human LILRA5 is a 258 amino acid (aa) type I transmembrane glycoprotein.  It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail.  LILRA5 has three potential splice forms.  One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299.  The other two splice forms are analogous to the above membrane and soluble forms, but possess a shortened signal sequence with an absence of aa 31-42.  Over aa 42-268, human LILRA5 shares 58% aa identity with mouse LILRA5.

Long Name
Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5
Entrez Gene IDs
353514 (Human); 232801 (Mouse); 691533 (Rat)
Alternate Names
CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7

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