Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.
Monoclonal Mouse IgG2A Clone # 711828
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ILT11/LILRA5 Gly42-Arg268 Accession # A6NI73
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 594
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human LILRA5/CD85f Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB6754T) or (B) Mouse IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
LILRA5, also known as Leukocyte Immunoglobulin-like Receptor 9 (LIR-9), CD85f, and ILT11, is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human LILRA5 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. LILRA5 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but possess a shortened signal sequence with an absence of aa 31-42. Over aa 42-268, human LILRA5 shares 58% aa identity with mouse LILRA5.
Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5
Entrez Gene IDs:
353514 (Human); 232801 (Mouse); 691533 (Rat)
CD85 antigen-like family member F; CD85; CD85f antigen; CD85F; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7
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