Human LILRA5/CD85f Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6754R-025
Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry.
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Human LILRA5/CD85f Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ILT11/LILRA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse ILT11/LILRA5, recombinant rat (rr) ILT11/LILRA5, rrLILRC2, recombinant human (rh) ILT4/CD85d, rhILT5/CD85a, or rhILT3/CD85k is observed.
Source
Monoclonal Mouse IgG2A Clone # 711828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ILT11/LILRA5
Gly42-Arg268
Accession # A6NI73
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LILRA5/CD85f antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRA5/CD85f Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB6754R) or (B) Mouse IgG2AAlexa Fluor 647 Isotype Control (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA5/CD85f

LILRA5, also known as Leukocyte Immunoglobulin-like Receptor 9 (LIR-9), CD85f, and ILT11, is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors.  It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta.  Mature human LILRA5 is a 258 amino acid (aa) type I transmembrane glycoprotein.  It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail.  LILRA5 has three potential splice forms.  One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299.  The other two splice forms are analogous to the above membrane and soluble forms, but possess a shortened signal sequence with an absence of aa 31-42.  Over aa 42-268, human LILRA5 shares 58% aa identity with mouse LILRA5.

Long Name
Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5
Entrez Gene IDs
353514 (Human); 232801 (Mouse); 691533 (Rat)
Alternate Names
CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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