Human LILRA5/CD85f Alexa Fluor® 700-conjugated Antibody Summary
Accession # A6NI73
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LILRA5/CD85f in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human LILRA5/CD85f Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB6754N) or (B) Mouse IgG2AAlexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
LILRA5, also known as Leukocyte Immunoglobulin-like Receptor 9 (LIR-9), CD85f, and ILT11, is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human LILRA5 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. LILRA5 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but possess a shortened signal sequence with an absence of aa 31-42. Over aa 42-268, human LILRA5 shares 58% aa identity with mouse LILRA5.
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