Human LILRB4/CD85k/ILT3 Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human ILT3/CD85k in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 293623
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ILT3/CD85k
    Accession # ABM86208
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LILRB4/CD85k/ILT3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL‑3 R alpha /CD123 APC‑conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB24251F) or (B) Mouse IgG2A Fluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LILRB4/CD85k/ILT3

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4
  • Entrez Gene IDs:
    11006 (Human); 14728 (Mouse)
  • Alternate Names:
    CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Protein kinase CK2 enables regulatory T cells to suppress excessive TH2 responses in vivo.
    Authors: Ulges A, Klein M, Reuter S, Gerlitzki B, Hoffmann M, Grebe N, Staudt V, Stergiou N, Bohn T, Bruhl T, Muth S, Yurugi H, Rajalingam K, Bellinghausen I, Tuettenberg A, Hahn S, Reissig S, Haben I, Zipp F, Waisman A, Probst H, Beilhack A, Buchou T, Filhol-Cochet O, Boldyreff B, Breloer M, Jonuleit H, Schild H, Schmitt E, Bopp T
    Nat Immunol, 2015;16(3):267-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011;6(7):e22504.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
    Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
    J. Leukoc. Biol., 2011;89(6):893-905.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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