Detects human LINGO-1 in direct ELISA and Western blot. In direct ELISA and Western blot, approximately 10% cross-reactivity with recombinant human (rh) LINGO-2 is observed and no cross-reactivity with rhLINGO-3 is observed.
Monoclonal Mouse IgG1 Clone # 332237
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human LINGO-1 Thr40-Thr556 Accession # NP_116197
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LINGO‑1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human LINGO‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB30861A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
LINGO-1 (LRRN6A) is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa sequence identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa sequence identity between human, mouse and rat.
Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1
Entrez Gene IDs:
FLJ14594; LERN1; LERN1UNQ201; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LINGO1; LINGO-1; LRRN6A; LRRN6Aleucine-rich repeat neuronal 6A; MGC17422
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