Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 332237
View all LINGO-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LINGO-1
Thr40-Thr556
Accession # NP_116197

Specificity

Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) LINGO-2 is observed and no cross-reactivity with rhLINGO-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LINGO‑1 Antibody

Detection of LINGO‑1 antibody in CHP‑100 Human Cell Line antibody by Flow Cytometry.

Detection of LINGO‑1 in CHP‑100 Human Cell Line by Flow Cytometry.

CHP-100 human neuro-blastoma cell line was stained with Mouse Anti-Human LINGO-1 Mono-clonal Antibody (Catalog # MAB30861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Applications for Human LINGO‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CHP‑100 human neuroblastoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed CHP-100 human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB30861 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LINGO-1

LINGO-1 (LRRN6A) is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa sequence identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa sequence identity between human, mouse and rat.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1

Alternate Names

LERN1, LINGO1, LRRN6A

Entrez Gene IDs

84894 (Human)

Gene Symbol

LINGO1

UniProt

NP_116197

Additional LINGO-1 Products

Product Documents for Human LINGO‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LINGO‑1 Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Human LINGO‑1 Antibody

Customer Reviews for Human LINGO‑1 Antibody (1)

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  • Human LINGO-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 06/25/2022
    Human LINGO‑1 Antibody MAB30861

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Protocols

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