Human LINGO-1 Antibody

Name: Human LINGO-1 Antibody
Brand: R&D Systems
SKU: MAB30861-SP
Rating: 5 (1 reviews)

Catalog #: MAB30861
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB30861
MAB30861-SP

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Detection of LINGO‑1 in CHP‑100 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LINGO-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) LINGO-2 is observed and no cross-reactivity with rhLINGO-3 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 332237

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LINGO-1
Thr40-Thr556
Accession # NP_116197

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed CHP-100 human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LINGO‑1 in CHP‑100 Human Cell Line by Flow Cytometry. CHP-100 human neuro-blastoma cell line was stained with Mouse Anti-Human LINGO-1 Mono-clonal Antibody (Catalog # MAB30861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LINGO-1

LINGO-1 (LRRN6A) is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily. The four known LINGO proteins contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa sequence identity. LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes. LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa sequence identity between human, mouse and rat.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1

Entrez Gene IDs

84894 (Human)

Alternate Names

FLJ14594; LERN1; LERN1UNQ201; leucine rich repeat and Ig domain containing 1; leucine rich repeat neuronal 6A; Leucine-rich repeat and immunoglobilin domain-containing protein 1; Leucine-rich repeat neuronal protein 1; Leucine-rich repeat neuronal protein 6A; LINGO1; LINGO-1; LRRN6A; LRRN6Aleucine-rich repeat neuronal 6A; MGC17422

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human LINGO-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

LINGO-1 protein interacts with the p75 neurotrophin receptor in intracellular membrane compartments.
Authors: Meabon J, De Laat R, Ieguchi K, Wiley J, Hudson M, Bothwell M
J Biol Chem, 2015-02-09;290(15):9511-20.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC