Human LPAR1/LPA₁/EDG-2 Antibody

Catalog #: MAB99631 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB99631-100
MAB99631-SP

Request a Quote Bulk Orders

Detection of LPAR1/LPA1/EDG-2 in Human PBMC Monocytes by Flow Cytometry.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LPAR1/LPA₁/EDG-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LPAR1/LPA₁/EDG-2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1020714

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LPAR1/LPA₁/EDG-2
Met1-Lys50
Accession # Q92633

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC Monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of LPAR1/LPA1/EDG-2 in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB99631, filled histogram) or Mouse IgG1 Isotype Control Antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name

Lysophosphatidic Acid Receptor 1

Entrez Gene IDs

1902 (Human); 14745 (Mouse)

Alternate Names

EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1