Human LPAR1/LPA1/EDG-2 Antibody (1020714)

R&D Systems | Catalog # MAB99631

R&D Systems
Discontinued Product
MAB99631 has been discontinued. View all LPAR1/LPA1/EDG-2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1020714
View all LPAR1/LPA1/EDG-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LPAR1/LPA1/EDG-2
Met1-Lys50
Accession # Q92633

Specificity

Detects human LPAR1/LPA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LPAR1/LPA1/EDG-2 Antibody (1020714)

Detection of LPAR1/LPA1/EDG-2 antibody in Human PBMC Monocytes antibody by Flow Cytometry.

Detection of LPAR1/LPA1/EDG-2 in Human PBMC Monocytes by Flow Cytometry.

Human PBMC monocytes were stained with Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB99631, filled histogram) or Mouse IgG1 Isotype Control Antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human LPAR1/LPA1/EDG-2 Antibody (1020714)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC Monocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Entrez Gene IDs

1902 (Human); 14745 (Mouse)

Gene Symbol

LPAR1

UniProt

Q92633

Additional LPAR1/LPA1/EDG-2 Products

Product Documents for Human LPAR1/LPA1/EDG-2 Antibody (1020714)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LPAR1/LPA1/EDG-2 Antibody (1020714)

For research use only

Citations for Human LPAR1/LPA1/EDG-2 Antibody (1020714)

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