Human LRRC32/GARP Alexa Fluor® 350-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRRC32/GARP in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse LRRC32 is observed.
  • Source
    Monoclonal Rat IgG2a Clone # 855151
  • Immunogen
    recombinant human LRRC32/GARP
    Accession # Q14392
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 350
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) with Anti-CD3, Anti-CD28, Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and Recombinant Human IL‑2 (Catalog # 202-IL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LRRC32/GARP
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature human LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine‑rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2, 3). Within the ECD, human LRRC32 shares approximately 80% aa sequence identity with mouse and rat LRRC32. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Human LRRC32 is identified as a lineage specific key receptor for human T cells. It is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6‑10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6‑8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
  • References:
    1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
    2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
    3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
    4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
    5. Macaulay, I.C. et al. (2007) Blood 109:3260.
    6. Wang, R. et al. (2008) PloS ONE 3:e2705.
    7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
    8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
    9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
    10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
    11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.
  • Long Name:
    Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
  • Entrez Gene IDs:
    2615 (Human); 434215 (Mouse)
  • Alternate Names:
    D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32
Related Research Areas
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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