Human LRRC32/GARP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRRC32/GARP in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse LRRC32 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 855151
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    recombinant human LRRC32/GARP
    Accession # Q14392
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LRRC32/GARP in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mLRecombinant Human IL‑2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human/Mouse LAP TGF‑ beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Mouse Anti-Human LRRC32/GARP Monoclonal Antibody (Catalog # MAB6055) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC32/GARP
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature human LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine‑rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2, 3). Within the ECD, human LRRC32 shares approximately 80% aa sequence identity with mouse and rat LRRC32. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Human LRRC32 is identified as a lineage specific key receptor for human T cells. It is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6‑10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6‑8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
  • References:
    1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
    2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
    3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
    4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
    5. Macaulay, I.C. et al. (2007) Blood 109:3260.
    6. Wang, R. et al. (2008) PloS ONE 3:e2705.
    7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
    8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
    9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
    10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
    11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.
  • Long Name:
    Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
  • Entrez Gene IDs:
    2615 (Human); 434215 (Mouse)
  • Alternate Names:
    D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32
Related Research Areas
Isotype Controls
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Rat IgG2A Isotype Control

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Secondary Antibodies
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

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APC-conjugated Antibody

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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

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Goat Anti-Rat IgG Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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