Human Lymphotoxin beta R/TNFRSF3 Alexa Fluor® 350-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB629U-100UG
R&D Systems Antibodies
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Lymphotoxin beta R/TNFRSF3 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, recombinant mouse (rm) Lymphotoxin  beta R, rhNGF R, rhOPG, rhRELT, rhTAJ, rhTNF RI or rhTNF RII is observed.
Source
Monoclonal Mouse IgG1 Clone # 71319
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
Gln31-Met227
Accession # P36941
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human whole blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References
  1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  2. Rennert, P.D. et al. (1998) Immunity 9:71.
  3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
  4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
  5. Crowe, P.D. et al. (1994) Science 264:707.
Long Name
Lymphotoxin beta Receptor
Entrez Gene IDs
4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)
Alternate Names
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Lymphotoxin beta R/TNFRSF3 Alexa Fluor® 350-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Lymphotoxin beta R/TNFRSF3 Alexa Fluor® 350-conjugated Antibody and earn rewards!

Have you used Human Lymphotoxin beta R/TNFRSF3 Alexa Fluor® 350-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review