Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, recombinant mouse (rm) Lymphotoxin beta R, rhNGF R, rhOPG, rhRELT, rhTAJ, rhTNF RI or rhTNF RII is observed.
Monoclonal Mouse IgG1 Clone # 71319
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3 Gln31-Met227 Accession # P36941
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Lymphotoxin beta R/TNFRSF3 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes (PBMCs) were stained with Mouse Anti-Human Lymphotoxin beta R/TNFRSF3 APC‑conjugated Monoclonal Antibody (Catalog # FAB629A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.
Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Rennert, P.D. et al. (1998) Immunity 9:71.
Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
Crowe, P.D. et al. (1994) Science 264:707.
Lymphotoxin beta Receptor
Entrez Gene IDs:
4055 (Human); 17000 (Mouse); 297604 (Rat)
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III
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