Human Lysosomal Pro-X Carboxypeptidase/PRCP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Lysosomal Pro‑X Carboxypeptidase/PRCP in ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 860821
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Lysosomal Pro‑X Carboxypeptidase/PRCP
    Met1-His496
    Accession # P42785
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Lysosomal Pro‑X Carboxypeptidase/PRCP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Lysosomal Pro‑X Carboxypeptidase/PRCP Monoclonal Antibody (Catalog # MAB7164) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lysosomal Pro‑X Carboxypeptidase/PRCP at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysosomal Pro-X Carboxypeptidase/PRCP

PRCP (Prolylcarboxypeptidase; also PCP, lysosomal Pro-X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57-62 kDa glycoprotein member of the S28 family of proteases. It is expressed by a number of cells including fibroblasts, macrophages, and endothelial cells. PRCP cleaves a variety of single C‑terminal amino acids (aa) adjacent to a Pro residue and is known to act on, and inactivate, peptides such as prekallikrein, alpha -MSH and angiotensin II plus III. Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa 1-21), a short prosequence (aa 22-45) and a 451 aa mature region (aa 46-496). The mature region demonstrates a Ser-carboxypeptidase domain (aa 55-477) that, in itself, possesses an SKS domain that caps the enzyme's catalytic site. The molecule is known to be mannosylated and to form noncovalent homodimers. There is one splice variant that contains a 21 aa insertion after Lys56. Over aa 1-496, human and mouse PRCP share 77% aa sequence identity.

  • Entrez Gene IDs:
    5547 (Human); 72461 (Mouse); 293118 (Rat)
  • Alternate Names:
    Angiotensinase C; EC 3.4.16.2; HUMPCP; Lysosomal carboxypeptidase C; lysosomal Pro-X carboxypeptidase; PCPMGC2202; PRCP; Proline carboxypeptidase; prolylcarboxypeptidase (angiotensinase C); prolylcarboxypeptidase isoform 1 preproprotein; Prolylcarboxypeptidase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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