Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 683715
View all MAdCAM-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MAdCAM-1
Leu21-Gln333
Accession # AAY82472

Specificity

Detects human MAdCAM-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse MAdCAM-1, recombinant human (rh) ALCAM, rhBCAM, rhCEACAM-1, rhEpCAM, rhICAM-1, -2, -3, -4, -5, rhCD31/PECAM-1, or rhVCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MAdCAM‑1 APC‑conjugated Antibody

Detection of MAdCAM‑1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of MAdCAM‑1 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MAdCAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB6056A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MAdCAM‑1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MAdCAM-1

Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins (1). Human MAdCAM-1 is synthesized as a 382 amino acid (aa) precursor that contains an 18 aa signal sequence, a 299 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there is one potential site for N-linked glycosylation (2). The ECD comprises two Ig-like domains of 90 aa and 119 aa, respectively, each possessing invariant cysteine residues that stabilize the Ig loop (2). There is also a Ser-Thr-Pro-rich (71%) mucin-like 48 aa domain that is (aa 206‑317) formed by six tandem repeats of an eight aa sequence having the general consensus DTTSPEP/SP. This mucin domain contains 19 potential sites for O-linked glycosylation (2, 3). A splicing variant in which a single Ala residue is substituted for aa 223‑334 in isoform 1 produces a second isoform. Human mature MAdCAM-1 shares only 44% aa sequence identity with mature mouse MAdCAM-1. The integrin alpha (4)  beta (7), which is expressed on lymphocytes, functions as the MAdCAM-1 receptor (1). The Ig domains of MAdCAM-1 are critical to alpha (4)  beta (7) binding, and the mucin domain has activity in L‑Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha  and IL‑1 beta. MAdCAM-1 is expressed on the surface of High Endothelial Venules (HEV) in the gut and in Peyer’s patches, on endothelial cells of the mesenteric lymph nodes, lamina propria of the small and large intestine, and the mammary gland during lactation, and on brain endothelial cells (1). MAdCAM‑1 has also been reported to be expressed in the liver portal region in autoimmune hepatitis (1), and in bone marrow following allogenic (genetically non-identical) hematopoietic stem cell transplantation, where it recruits donor T cells, which may lead to graft versus host disease (3, 4). MAdCAM‑1 functions as a homing receptor, and plays a central role in leukocyte migration into HEVs and Peyer’s patch (5). In addition to its normal role in lymphocyte trafficking to mucosal tissue, MAdCAM‑1 expression is also dramatically increased in chronic inflammatory and disease states (1, 6), including inflammatory bowel disease (Crohn’s disease and ulcerative colitis) (7), sclerosing cholangitis (8), and diabetes (9), and may play an important role in these conditions.

References

  1. Ando, T. et al. (2007) BMC Physiol. 7:10.
  2. Dando, J. et al. (2002) Acta Crystallogr. D 58:233.
  3. Leung, E. et al. (1996) Immunol. Cell Biol. 74:490.
  4. Ambruzova, Z. et al. (2009) Hum. Immunol. 70:457.
  5. Tada, T. et al. (2008) Exp. Anim. 57:247.
  6. Volpes, R. et al. (1992) Hepatology 15:269.
  7. Connor, E.M. et al. (1999) J. Leukoc. Biol. 65:349.
  8. Ala, A. et al. (2001) Gut 49:3043.
  9. Yang, X.D. et al. (1997) Diabetes 46:1542.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Gene Symbol

MADCAM1

UniProt

AAY82472

Additional MAdCAM-1 Products

Product Documents for Human MAdCAM‑1 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MAdCAM‑1 APC‑conjugated Antibody

For research use only

Citations for Human MAdCAM‑1 APC‑conjugated Antibody

Customer Reviews for Human MAdCAM‑1 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human MAdCAM‑1 APC‑conjugated Antibody and earn rewards!

Have you used Human MAdCAM‑1 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies