Human MAP4K5 Antibody

Catalog # Availability Size / Price Qty
AF8007
AF8007-SP
Detection of Human MAP4K5 by Western Blot.
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Human MAP4K5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MAP4K5 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MAP4K5
Lys395-Asn484 (Asn473Lys)
Accession # Q9Y4K4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human MAP4K5 by Western Blot. View Larger

Detection of Human MAP4K5 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MAP4K5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAP4K5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MAP4K5

MAP4K5 (MAP Kinase Kinase Kinase Kinase 5; also known as KHS, MEKKK5, and GCKR) is a 94-96 kDa member of the STE (STErile 20 protein) subfamily, STE Ser/Thr protein kinase family, protein kinase superfamily of enzymes. It is ubiquitously expressed, and serves as an "intermediary" between GPCRs and cytosolic MAP kinases. It also interacts with Crk (CT10 Regulator of Kinase)-related adaptor molecules, facilitating the formation of signaling complexes around Tyr phosphorylated molecules. And finally, it represents an upstream component of a TNFR1 signaling pathway that requires activation of stress-activated protein kinases. Human MAP4K5 is 846 amino acids 9aa) in length. It contains a 258 aa kinase domain (aa 20-277) that is coupled to a 316 aa CNH (citron homology) domain (aa 506-826) which typically binds GTPases. There are two isoform variants. One shows a deletion of both Pro481 and Glu569, while another possesses a substitution of 16 aa for aa 569-846. Over aa 395-484, human MAP4K5 shares 83% aa sequence identity with mouse MAP4K5.

Long Name
Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase
Entrez Gene IDs
11183 (Human); 399510 (Mouse); 503027 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; GCKR; germinal center kinase-related; KHS1; KHSMEKKK 5; Kinase homologous to SPS1/STE20; MAP4K5; MAPK/ERK kinase kinase kinase 5; MAPKKKK5; MEK kinase kinase 5; mitogen-activated protein kinase kinase kinase kinase 5

Product Datasheets

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Human MAP4K5 Antibody
By Anonymous on 04/26/2017
Application: WB Sample Tested: Cell Lysates Species: Human