Human MFG-E8 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human MFG-E8 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 278918
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MFG-E8
    Accession # Q08431
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of MFG‑E8 in Human Dendritic Cells by Flow Cytometry. Human dendritic cells were stained with Mouse Anti-Human MFG‑E8 PE‑conjugated Monoclonal Antibody (Catalog # IC27671P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MFG-E8
Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).
  • References:
    1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
    2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
    3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
    4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
    5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
    6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
    7. Hanayama, R. et al. (2002) Nature 417:182.
    8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
    9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
    10. Hanayama, R. et al. (2004) Science 304:1147.
    11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
    12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
    13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
    14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
    15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
  • Long Name:
    Milk Fat Globule EGF Factor 8
  • Entrez Gene IDs:
    4240 (Human); 17304 (Mouse)
  • Alternate Names:
    BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 10  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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