Human MFG-E8 Antibody

Catalog # Availability Size / Price Qty
MAB27671
MAB27671-SP
Detection of MFG‑E8 in Human Immature Dendritic Cells by Flow Cytometry.
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Product Details
Citations (4)
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Reviews (1)

Human MFG-E8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MFG-E8 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 278918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MFG-E8
Leu24-Cys387
Accession # Q08431
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MFG-E8 (Catalog # 2767-MF) under non-reducing conditions only
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human immature dendritic cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and TF-1 cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of MFG-E8 antibody in Human Immature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of MFG‑E8 in Human Immature Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Mouse Anti-Human MFG-E8 Monoclonal Antibody (Catalog # MAB27671, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry View Larger

Detection of MFG‑E8 in TF-1 cells by Flow Cytometry. TF-1 cells were stained with Mouse Anti-Human MFG‑E8 Monoclonal Antibody (Catalog # MAB27671, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with FC012 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFG-E8

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).

References
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
  3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
  4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
  5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  7. Hanayama, R. et al. (2002) Nature 417:182.
  8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  10. Hanayama, R. et al. (2004) Science 304:1147.
  11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Long Name
Milk Fat Globule EGF Factor 8
Entrez Gene IDs
4240 (Human); 17304 (Mouse)
Alternate Names
BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47

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Citations for Human MFG-E8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Development of human cell-expressed tag-free rhMFG-E8 as a radiation mitigator and a therapeutic for acute kidney injury
    Authors: Chaung, W;Zhang, F;Ma, G;Yen, H;Jacob, A;Brenner, M;Wang, P;
    Research square
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Complex Application
  2. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node
    Authors: Chung Park, John H Kehrl
    eLife
  3. Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies
    Authors: BJ Benedikter, FG Bouwman, T Vajen, ACA Heinzmann, G Grauls, EC Mariman, EFM Wouters, PH Savelkoul, C Lopez-Igle, RR Koenen, GGU Rohde, FRM Stassen
    Sci Rep, 2017-11-10;7(1):15297.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Milk fat globule EGF-8 promotes melanoma progression through coordinated Akt and twist signaling in the tumor microenvironment.
    Authors: Jinushi M, Nakazaki Y, Carrasco DR, Draganov D, Souders N, Johnson M, Mihm MC, Dranoff G
    Cancer Res., 2008-11-01;68(21):8889-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human MFG-E8 Antibody
By Adam Guess on 08/01/2016
Application: Flow Sample Tested: Bone marrow cells Species: Human