Human MICA/B Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB13001N-100
FAB13001N-025
Human MICA/B Alexa Fluor® 700-conjugated Antibody in Data
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Human MICA/B Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA/B in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 159207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MICA/B antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB13001N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name
MHC Class I-related Protein A and B
Entrez Gene IDs
4276 (Human)
Alternate Names
MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICA/B; MICB; PERB11; PERB11.1; PERB11.2

Product Datasheets

FAQs

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Human MICA/B Alexa Fluor® 700-conjugated Antibody
By Anonymous on 07/05/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human