Human MICA/B Antibody

Catalog #: MAB13001
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13001-SP
MAB13001-500
MAB13001-100

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Detection of MICA/B in K562 Human Cell Line by Flow Cytometry.

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Product Details

Citations (12)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA/B Antibody Summary

Species Reactivity

Human

Specificity

Detects human MICA/B in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 159207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Biotinylated Human MICA Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299‑NK) coated at 2 µg/mL (100 µL/well). At 0.4 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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MICA/B in Human Pancreas. MICA/B was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name

MHC Class I-related Protein A and B

Entrez Gene IDs

4276 (Human)

Alternate Names

MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICA/B; MICB; PERB11; PERB11.1; PERB11.2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Natural Killer T (NKT) Cells

Citations for Human MICA/B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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The metabolism of cells regulates their sensitivity to NK cells depending on p53 status
Authors: S Belkahla, JM Brualla, A Fayd'herbe, P Falvo, N Allende-Ve, M Constantin, AUH Khan, L Coenon, C Alexia, G Mitola, P Massa, S Orecchioni, F Bertolini, W Mnif, J Hernandez, A Anel, M Villalba
Scientific Reports, 2022;12(1):3234.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
Authors: N Allende-Ve, J Marco Brua, P Falvo, C Alexia, M Constantin, AF de Maudave, L Coenon, D Gitenay, G Mitola, P Massa, S Orecchioni, F Bertolini, I Marzo, A Anel, M Villalba
Scientific Reports, 2022;12(1):1341.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Exploiting the DNA Damaging Activity of Liposomal Low Dose Cytarabine for Cancer Immunotherapy
Authors: JD Lewicky, AL Martel, NL Fraleigh, E Picard, L Mousavifar, A Nakamura, F Diaz-Mitom, R Roy, HT Le
Pharmaceutics, 2022;14(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Toll-like Receptor-4 Activation Boosts the Immunosuppressive Properties of Tumor Cells-derived Exosomes
Authors: R Domenis, A Cifù, D Marinò, M Fabris, KR Niazi, P Soon-Shion, F Curcio
Sci Rep, 2019;9(1):8457.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Lower protein expression levels of MHC class I chain-related gene A in hepatocellular carcinoma are at high risk of recurrence after surgical resection
Authors: CF Huang, SC Wang, WT Chang, ML Yeh, CI Huang, ZY Lin, SC Chen, WL Chuang, JF Huang, CY Dai, YL Chen, ML Yu
Sci Rep, 2018;8(1):15821.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
Eur J Immunol, 2015;45(8):2324-34.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation
Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis.
Authors: Kim H, Bose S, Meyer K, Ray R
J Virol, 2014;88(5):2564-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
PLoS Pathog., 2012;8(1):e1002486.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
Oncogene, 2011;30(23):2622-32.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
PLoS ONE, 2011;6(2):e16899.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
Proc. Natl. Acad. Sci. U.S.A., 2006;103(30):11258-63.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
Cancer Res., 2005;65(23):11136-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry