Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, FACS

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 159207
View all MICA/B Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983

Specificity

Detects human MICA/B in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human MICA/B PE‑conjugated Antibody

Detection of MICA/B antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B PE-conjugated Monoclonal Antibody (Catalog # FAB13001P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MICA/B PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name

MHC Class I-related Protein A and B

Alternate Names

MHC class I chain-related protein A, MHC class I chain-related protein B, MIC, MICA, MICB, PERB11, PERB11.1, PERB11.2

Entrez Gene IDs

4276 (Human)

Gene Symbol

MICA

UniProt

Q29983

Additional MICA/B Products

Product Documents for Human MICA/B PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MICA/B PE‑conjugated Antibody

For research use only

Related Research Areas

Natural Killer T (NKT) Cells

Citations for Human MICA/B PE‑conjugated Antibody

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FAQs

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