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Human MLK3 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4559S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4559S-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MLK3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human MLK3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MLK3
Ala85-Gly207
Accession # Q16584
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MLK3

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha, and ERK1.

Long Name
Mixed Lineage Kinase 3
Entrez Gene IDs
4296 (Human); 26403 (Mouse); 309168 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.25; MAP3K11; MEKK11; mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK3; MLK3MLK-3; PTK1; PTK1protein-tyrosine kinase PTK1; RHOE; SH3 domain-containing proline-rich kinase; SPRK; SPRKMGC17114; Src-homology 3 domain-containing proline-rich kinase

Product Datasheets

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