Human MLK3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human MLK3 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MLK3
    Ala85-Gly207
    Accession # Q16584
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MLK3 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK3 at approximately 93 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLK3

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha, and ERK1.

  • Long Name:
    Mixed Lineage Kinase 3
  • Entrez Gene IDs:
    4296 (Human); 26403 (Mouse); 309168 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.25; MAP3K11; MEKK11; mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK3; MLK3MLK-3; PTK1; PTK1protein-tyrosine kinase PTK1; RHOE; SH3 domain-containing proline-rich kinase; SPRK; SPRKMGC17114; Src-homology 3 domain-containing proline-rich kinase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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