Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MLK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MLK3
Ala85-Gly207
Accession # Q16584

Specificity

Detects endogenous human MLK3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MLK3 Antibody

Detection of Human MLK3 antibody by Western Blot.

Detection of Human MLK3 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK3 at approximately 93 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human MLK3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MLK3

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha, and ERK1.

Long Name

Mixed Lineage Kinase 3

Alternate Names

MAP3K11, PTK1, RHOE, SPRK

Entrez Gene IDs

4296 (Human); 26403 (Mouse); 309168 (Rat)

Gene Symbol

MAP3K11

UniProt

Q16584

Additional MLK3 Products

Product Documents for Human MLK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MLK3 Antibody

For research use only

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FAQs

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