Human MMP-14/MT1-MMP Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
  • Source
    Monoclonal Mouse IgG2B Clone # 128527
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
    Tyr112-Ala541 (predicted)
    Accession # P50281
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB9181N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-14/MT1-MMP

MMP-14/MT1-MMP (Matrix Metalloproteinase 14) is a member of the peptidase M10A family of enzymes. As such, it is one of some 25 zinc-dependent endopeptidases that are either soluble or membrane-bound.  MMP-14 is expressed as a 63-64 kDa precursor that undergoes processing to generate either a 60 kDa catalytically-active transmembrane form, or a 55 kDa active soluble form. A number of substrates are reported for MMP-14, including Collagen I, II, and III, Laminin, Endoglin, Muc-1, Pro-MMP-13 and -2, and CD44. Molecules suggested to inhibit MMP-14 include TIMP-2, -3, and -4. Cells known to express MMP-14 include monocytes, platelets, osteoclasts, endothelial cells, fibroblasts, and muscle satellite cells. Over amino acids (aa) 112-284, human MMP-14 shares 98% aa sequence identity with mouse MMP-14.

  • Long Name:
    Matrix Metalloproteinase 14/Membrane Type 1 MMP
  • Entrez Gene IDs:
    4323 (Human)
  • Alternate Names:
    EC 3.4.24; EC; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC0041N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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