Human MMP-14/MT1-MMP APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
  • Source
    Monoclonal Mouse IgG2B Clone # 128527
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP
    Tyr112-Ala541 (predicted)
    Accession # P50281
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP APC-conjugated Monoclonal Antibody (Catalog # FAB9181A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-14/MT1-MMP

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).

  • References:
    1. Seike, M. (2003) Cancer Lett. 194:1.
    2. Sato, H. et al. (1994) Nature 370:61.
  • Long Name:
    Matrix Metalloproteinase 14/Membrane Type 1 MMP
  • Entrez Gene IDs:
    4323 (Human)
  • Alternate Names:
    EC 3.4.24; EC; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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