Detection of MMP‑14/MT1‑MMP in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9181G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
MMP-14/MT1-MMP (Matrix Metalloproteinase 14) is a member of the peptidase M10A family of enzymes. As such, it is one of some 25 zinc-dependent endopeptidases that are either soluble or membrane-bound. MMP-14 is expressed as a 63-64 kDa precursor that undergoes processing to generate either a 60 kDa catalytically-active transmembrane form, or a 55 kDa active soluble form. A number of substrates are reported for MMP-14, including Collagen I, II, and III, Laminin, Endoglin, Muc-1, Pro-MMP-13 and -2, and CD44. Molecules suggested to inhibit MMP-14 include TIMP-2, -3, and -4. Cells known to express MMP-14 include monocytes, platelets, osteoclasts, endothelial cells, fibroblasts, and muscle satellite cells. Over amino acids (aa) 112-284, human MMP-14 shares 98% aa sequence identity with mouse MMP-14.
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