Detects human MMP-9 in Western blots. In Western blots, reactivity with the pro (92 kDa), active (82 kDa), and C-terminal truncated (65 kDa) forms of recombinant human (rh) MMP-9 is observed. Also in Western blots, 20% cross-reactivity with rhMMP-2, 5% cross‑reactivity with rhMMP-1, and no cross-reactivity with rhMMP-3, -7, -8, -10, -12, or -13 is observed.
Monoclonal Mouse IgG1 Clone # 4H3
Protein A or G purified from ascites
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immersion fixed paraffin-embedded sections of human ovarian and breast cancer tissues
Detection of Human MMP‑9 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP‑9 Monoclonal Antibody (Catalog # MAB911) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑9 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
metalloproteinases are a family of zinc and calcium dependent
endopeptidases with the combined ability to degrade all the components
of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad
range of substrates including gelatin, collagen types IV and V, elastin
and proteoglycan core protein. It is believed to act synergistically
with interstitial collagenase (MMP-1) in the degradation of fibrillar
collagens as it degrades their denatured gelatin forms. MMP-9 is
produced by keratinocytes, monocytes, macrophages and PMN leukocytes.
MMP-9 is present in most cases of inflammatory responses. Structurally,
MMP-9 maybe be divided into five distinct domains: a pro-domain which is
cleaved upon activation, a gelatin-binding domain consisting of three
contiguous fibronectin type II units, a catalytic domain containing the
zinc binding site, a proline-rich linker region, and a carboxyl terminal
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 22.214.171.124; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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