Human MMP-9 Antibody
Human MMP-9 Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MMP‑9 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-9 Monoclonal Antibody (Catalog # MAB911) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-9 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
Product Datasheets
Citations for Human MMP-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker
Authors: M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström
Cancers, 2022;14(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Ameliorating Fibrosis in Murine and Human Tissues with END55, an Endostatin-Derived Fusion Protein Made in Plants
Authors: L Mlakar, SM Garrett, T Watanabe, M Sanderson, T Nishimoto, J Heywood, KL Helke, JM Pilewski, EL Herzog, C Feghali-Bo
Biomedicines, 2022;10(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
JNK and p38 Inhibitors Prevent Transforming Growth Factor-&beta1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts
Authors: TY Hou, SB Wu, HC Kau, CC Tsai
International Journal of Molecular Sciences, 2021;22(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
Nat Commun, 2014;5(0):4255.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
Infect. Immun., 2010;78(3):1012-21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biol. Reprod., 2010;82(5):968-79.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA -
Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2009;6(1):9-17.
Species: Human
Sample Types: Tissue Secretion
Applications: Western Blot -
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
Metab. Clin. Exp., 2009;58(7):946-53.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Authors: Lucio-Eterovic AK, Piao Y, de Groot JF
Clin. Cancer Res., 2009;15(14):4589-99.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
Int. J. Cancer, 2008;122(12):2805-10.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma.
Authors: Kleinman ME, Greives MR, Churgin SS, Blechman KM, Chang EI, Ceradini DJ, Tepper OM, Gurtner GC
Arterioscler. Thromb. Vasc. Biol., 2007;27(12):2664-70.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2007;178(9):5957-65.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
Arthritis Rheum., 2007;56(10):3326-35.
Species: Human
Sample Types: Synovial Fluid
Applications: Immunoprecipitation -
Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
Surgery, 2006;140(3):454-63.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut, 2006;55(12):1774-80.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
Authors: Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
Laryngoscope, 2005;115(1):56-61.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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