Human MMP-9 Antibody

Catalog # Availability Size / Price Qty
MAB911
MAB911-SP
Human MMP-9 Antibody in Western Blot
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Product Details
Citations (13)
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Human MMP-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-9 in Western blots. In Western blots, reactivity with the pro (92 kDa), active (82 kDa), and C-terminal truncated (65 kDa) forms of recombinant human (rh) MMP-9 is observed. Also in Western blots, 20% cross-reactivity with rhMMP-2, 5% cross‑reactivity with rhMMP-1, and no cross-reactivity with rhMMP-3, -7, -8, -10, -12, or -13 is observed.
Source
Monoclonal Mouse IgG1 Clone # 4H3
Purification
Protein A or G purified from ascites
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
25-100 µg/mL
Immersion fixed paraffin-embedded sections of human ovarian and breast cancer tissues
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
CyTOF-reported
Brodie, T.M. et al. (2018) Cytometry PartA. 93: 406. Ready to be labeled using establishedconjugation methods. No BSA or other carrier proteins that could interfere withconjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human MMP‑9 by Western Blot. View Larger

Detection of Human MMP‑9 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 5 ng/mL PMA for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-9 Monoclonal Antibody (Catalog # MAB911) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-9 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Product Datasheets

Citations for Human MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
    Authors: Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
    Nat Commun, 2014;5(0):4255.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
    Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
    Infect. Immun., 2010;78(3):1012-21.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  4. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2009;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  5. Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
    Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
    Metab. Clin. Exp., 2009;58(7):946-53.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  6. Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
    Authors: Lucio-Eterovic AK, Piao Y, de Groot JF
    Clin. Cancer Res., 2009;15(14):4589-99.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
    Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
    Int. J. Cancer, 2008;122(12):2805-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Hypoxia-induced mediators of stem/progenitor cell trafficking are increased in children with hemangioma.
    Authors: Kleinman ME, Greives MR, Churgin SS, Blechman KM, Chang EI, Ceradini DJ, Tepper OM, Gurtner GC
    Arterioscler. Thromb. Vasc. Biol., 2007;27(12):2664-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007;178(9):5957-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Neutrophil gelatinase-associated lipocalin is expressed in osteoarthritis and forms a complex with matrix metalloproteinase 9.
    Authors: Gupta K, Shukla M, Cowland JB, Malemud CJ, Haqqi TM
    Arthritis Rheum., 2007;56(10):3326-35.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: Immunoprecipitation
  11. Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
    Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
    Surgery, 2006;140(3):454-63.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  12. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006;55(12):1774-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
    Authors: Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
    Laryngoscope, 2005;115(1):56-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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