Human MMP-9 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry.
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Product Details
Citations (7)
Supplemental Products
Reviews (2)

Human MMP-9 Fluorescein-conjugated Antibody Summary

Species Reactivity
Detects human MMP-9 in Flow Cytometry.
Monoclonal Mouse IgG2B Clone # 56129
Protein A or G purified from ascites
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of MMP-9 antibody in NS0 Mouse Cell Line Transfected with Human MMP-9 antibody by Flow Cytometry. View Larger

Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP-9 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Human MMP-9 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study
    Authors: C Jiguet-Jig, S Boissonnea, E Denicolai, V Hein, R Lasseur, J Garcia, S Romain, R Appay, T Graillon, W Mason, AF Carpentier, AA Brandes, L' Ouafik, W Wick, A Baaziz, JP Gigan, RJ Argüello, D Figarella-, O Chinot, E Tabouret
    Acta neuropathologica communications, 2022;10(1):1.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Amphiregulin Regulates Phagocytosis-Induced Cell Death in Monocytes via EGFR and Matrix Metalloproteinases
    Authors: C Platen, S Dreschers, LK Reiss, J Wappler, TW Orlikowsky
    Mediators Inflamm., 2018;2018(0):4310419.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells
    Authors: C Lavini-Ram, HM Silva, A Soares-Sch, SM Monteiro, LR Ferreira, AP Pacanaro, S Gomes, J Batista, K Faé, J Kalil, V Coelho
    Sci Rep, 2017;7(1):874.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease
    Authors: NI Medeiros, RC Fares, EP Franco, GR Sousa, RT Mattos, AT Chaves, MD Nunes, WO Dutra, R Correa-Oli, MO Rocha, JA Gomes
    PLoS Negl Trop Dis, 2017;11(1):e0005284.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity
    Authors: PR Taylor, S Roy, EC Meszaros, Y Sun, SJ Howell, CJ Malemud, E Pearlman
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia.
    Authors: Walsby E, Buggins A, Devereux S, Jones C, Pratt G, Brennan P, Fegan C, Pepper C
    Blood, 2014;123(23):3607-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human MMP-9 Fluorescein-conjugated Antibody
By Christian Ostalecki on 06/08/2018
Application: ICC/IF Sample Tested: Alzheimer's disease brain Species: Human

Human MMP-9 Fluorescein-conjugated Antibody
By Lucy Quach on 04/02/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line,HT-29 human colon adenocarcinoma cell line Species: Human