Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry.
NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
metalloproteinases are a family of zinc and calcium dependent
endopeptidases with the combined ability to degrade all the components
of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad
range of substrates including gelatin, collagen types IV and V, elastin
and proteoglycan core protein. It is believed to act synergistically
with interstitial collagenase (MMP-1) in the degradation of fibrillar
collagens as it degrades their denatured gelatin forms. MMP-9 is
produced by keratinocytes, monocytes, macrophages and PMN leukocytes.
MMP-9 is present in most cases of inflammatory responses. Structurally,
MMP-9 maybe be divided into five distinct domains: a pro-domain which is
cleaved upon activation, a gelatin-binding domain consisting of three
contiguous fibronectin type II units, a catalytic domain containing the
zinc binding site, a proline-rich linker region, and a carboxyl terminal
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 220.127.116.11; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Human MMP-9 Fluorescein-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you
include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image.
Limited to verified customers in USA, Canada and Europe.
Read what people are saying who have used Human MMP-9 Fluorescein-conjugated Antibody.
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.
Filter by Ratings
Filter by Applications
Filter by Species
HCT-116 human colorectal carcinoma cell line,HT-29 human colon adenocarcinoma cell line