Human MMP‑9 Fluorescein‑conjugated Antibody

R&D Systems | Catalog # IC9111F

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 56129
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-9

Specificity

Detects human MMP-9 in Flow Cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MMP‑9 Fluorescein‑conjugated Antibody

Detection of MMP‑9 in U2OS cells treated with 100ng/mL PMA for 24 hrs by Flow Cytometry

Serum starved U2OS cells treated with 100ng/mL PMA for 24 hrs (filled histogram) vs untreated U2OS cells (open histogram) were stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human MMP‑9 Fluorescein‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: U2OS human osteosarcoma cell line treated with 100ng/mL PMA for 24 hrs fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 2 reviews rated 3 using IC9111F in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Human MMP‑9 Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP‑9 Fluorescein‑conjugated Antibody

For research use only

Citations for Human MMP‑9 Fluorescein‑conjugated Antibody

Customer Reviews for Human MMP‑9 Fluorescein‑conjugated Antibody (2)

3 out of 5
2 Customer Ratings
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  • Human MMP-9 Fluorescein-conjugated Antibody
    Name: Christian Ostalecki
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Alzheimer's disease brain
    Species: Human
    Verified Customer | Posted 06/08/2018
    Human MMP‑9 Fluorescein‑conjugated Antibody IC9111F
  • Human MMP-9 Fluorescein-conjugated Antibody
    Name: Lucy Quach
    Application: Flow Cytometry
    Sample Tested: HCT-116 human colorectal carcinoma cell line and HT-29 human colon adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 04/02/2018

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