Human MMP-9 Fluorescein-conjugated Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human MMP-9 in Flow Cytometry.
  • Source
    Monoclonal Mouse IgG2B Clone # 56129
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-9
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.
  • Long Name:
    Matrix Metalloproteinase 9
  • Entrez Gene IDs:
    4318 (Human); 17395 (Mouse); 81687 (Rat)
  • Alternate Names:
    92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Differential Expression of Matrix Metalloproteinases 2, 9 and Cytokines by Neutrophils and Monocytes in the Clinical Forms of Chagas Disease
    Authors: NI Medeiros, RC Fares, EP Franco, GR Sousa, RT Mattos, AT Chaves, MD Nunes, WO Dutra, R Correa-Oli, MO Rocha, JA Gomes
    PLoS Negl Trop Dis, 2017;11(1):e0005284.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. MMP9 integrates multiple immunoregulatory pathways that discriminate high suppressive activity of human mesenchymal stem cells
    Authors: C Lavini-Ram, HM Silva, A Soares-Sch, SM Monteiro, LR Ferreira, AP Pacanaro, S Gomes, J Batista, K Faé, J Kalil, V Coelho
    Sci Rep, 2017;7(1):874.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity
    Authors: PR Taylor, S Roy, EC Meszaros, Y Sun, SJ Howell, CJ Malemud, E Pearlman
    J Leukoc Biol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Development and characterization of a physiologically relevant model of lymphocyte migration in chronic lymphocytic leukemia.
    Authors: Walsby E, Buggins A, Devereux S, Jones C, Pratt G, Brennan P, Fegan C, Pepper C
    Blood, 2014;123(23):3607-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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