Detection of MMP‑9 in NS0 Mouse Cell Line Transfected with Human MMP-9 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human MMP-9 was stained with Mouse Anti-Human MMP‑9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC9111F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
metalloproteinases are a family of zinc and calcium dependent
endopeptidases with the combined ability to degrade all the components
of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad
range of substrates including gelatin, collagen types IV and V, elastin
and proteoglycan core protein. It is believed to act synergistically
with interstitial collagenase (MMP-1) in the degradation of fibrillar
collagens as it degrades their denatured gelatin forms. MMP-9 is
produced by keratinocytes, monocytes, macrophages and PMN leukocytes.
MMP-9 is present in most cases of inflammatory responses. Structurally,
MMP-9 maybe be divided into five distinct domains: a pro-domain which is
cleaved upon activation, a gelatin-binding domain consisting of three
contiguous fibronectin type II units, a catalytic domain containing the
zinc binding site, a proline-rich linker region, and a carboxyl terminal
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 188.8.131.52; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
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