Human/Mouse AlphaA Crystallin/CRYAA Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4848V Datasheet / COA / SDS

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AF4848V-100UG

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Human/Mouse AlphaA Crystallin/CRYAA Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AlphaA Crystallin/CRYAA
Met1-Ser173
Accession # P02489

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AlphaA Crystallin/CRYAA

AlphaA Crystallin (CRYAA, also known as Crystallin alpha -A chain and HspB4) is a 21‑22 kDa member of the HSP20 family of proteins. It is found in lens epithelium where it noncovalently oligomerizes with CRY-alpha B to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAA is 173 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 63‑145. Multiple posttranslational modifications may exist. The C-terminal Ser and the N-terminal MetAspVal is occasionally cleaved. There is also phosphorylation at Ser45 and 122, O-linked GlcNAc modification at Ser162, deamidation at Asn101, and acetylation at Lys70. Full‑length human CRYAA is 54% aa identical to CRY-AlphaB, and 95% aa identical to mouse CRYAA.

Entrez Gene IDs

1409 (Human); 12954 (Mouse)

Alternate Names

AlphaA Crystallin; CRYA1; CRYA1crystallin, alpha-1; CRYAA; crystallin, alpha A; Heat shock protein beta-4; HSPB4; HSPB4alpha-crystallin A chain; human alphaA-crystallin (CRYA1), 10HspB4