AlphaA Crystallin (CRYAA, also known as Crystallin alpha -A chain and HspB4) is a 21‑22 kDa member of the HSP20 family of proteins. It is found in lens epithelium where it noncovalently oligomerizes with CRY-alpha B to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAA is 173 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 63‑145. Multiple posttranslational modifications may exist. The C-terminal Ser and the N-terminal MetAspVal is occasionally cleaved. There is also phosphorylation at Ser45 and 122, O-linked GlcNAc modification at Ser162, deamidation at Asn101, and acetylation at Lys70. Full‑length human CRYAA is 54% aa identical to CRY-AlphaB, and 95% aa identical to mouse CRYAA.
Human/Mouse AlphaA Crystallin/CRYAA Antibody
R&D Systems | Catalog # AF4848
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human AlphaA Crystallin/CRYAA
Met1-Ser173
Accession # P02489
Met1-Ser173
Accession # P02489
Specificity
Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse AlphaA Crystallin/CRYAA Antibody
Detection of Human/Mouse AlphaA Crystallin/CRYAA by Western Blot.
Western blot shows lysates of mouse eye lens tissue. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. Bands for AlphaA Crystallin/CRYAA were detected at approximately 20 - 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Mouse AlphaA Crystallin/CRYAA by Simple WesternTM.
Simple Western lane view shows lysates of mouse eye lens tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaA Crystallin/CRYAA at approximately 28 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse AlphaA Crystallin/CRYAA Antibody
Application
Recommended Usage
Simple Western
1 µg/mL
Sample: Mouse eye lens tissue
Sample: Mouse eye lens tissue
Western Blot
0.1 µg/mL
Sample: Mouse eye lens tissue
Sample: Mouse eye lens tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: AlphaA Crystallin/CRYAA
Alternate Names
CRYA1, CRYAA, HSPB4
Gene Symbol
CRYAA
UniProt
Additional AlphaA Crystallin/CRYAA Products
Product Documents for Human/Mouse AlphaA Crystallin/CRYAA Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse AlphaA Crystallin/CRYAA Antibody
For research use only
Related Research Areas
Citations for Human/Mouse AlphaA Crystallin/CRYAA Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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