Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 290728
View all AMIGO2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse AMIGO2
Met39-Ala391
Accession # Q80ZD9

Specificity

Detects mouse AMIGO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) AMIGO2 and no cross-reactivity with rhAMIGO1 or recombinant mouse AMIGO3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse AMIGO2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human and mouse AMIGO2

Reviewed Applications

Read 2 reviews rated 3 using MAB2374 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMIGO2

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

Long Name

Amphoterin-induced Gene and ORF 2

Alternate Names

Alivin-1

Entrez Gene IDs

347902 (Human); 105827 (Mouse); 102124161 (Cynomolgus Monkey)

Gene Symbol

AMIGO2

UniProt

Q80ZD9

Additional AMIGO2 Products

Product Documents for Human/Mouse AMIGO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse AMIGO2 Antibody

For research use only

Customer Reviews for Human/Mouse AMIGO2 Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Showing  1 - 2 of 2 reviews Showing All
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  • Human/Mouse AMIGO2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human colon cancer tissue
    Species: Human
    Verified Customer | Posted 07/20/2022
    1, Amigo2 antibody, clone:290728, MAB2374, lot:VLF015051, rat mAB, IgG2a, 100µg/200µl PBS, 0,5mg/ml, R&D 2, The tissue is human colon cancer tissue 3, The Amigo2 antibody dilution is 1 to 50, 1 to 250, but it does not work
    Human/Mouse AMIGO2 Antibody MAB2374
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Human/Mouse AMIGO2 Antibody
    Name: Kathleen Maguire-Zeiss
    Application: Immunocytochemistry
    Sample Tested: primary astrocytes
    Species: Mouse
    Verified Customer | Posted 10/05/2017
    Mouse Primary Coritcal Astrocytes Stained with MAB2374 and DAPI
    Human/Mouse AMIGO2 Antibody MAB2374

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