Human/Mouse DACT3/Dapper 3 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF5767S Datasheet / COA / SDS

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AF5767S-100UG

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Human/Mouse DACT3/Dapper 3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse DACT3/Dapper 3
Pro486-Lys587
Accession # Q0PHV7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DACT3/Dapper 3

Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins. It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta -catenin signaling. When present in colorectal tumor cells, it initiates apoptosis. Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta -catenin entry into the nucleus. Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610). There is one potential splice variant that shows a 45 aa substitution for aa 413-610. In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.

Long Name

Dapper, Antagonist of beta-Catenin, Homolog 3

Entrez Gene IDs

147906 (Human); 629378 (Mouse); 499088 (Rat)

Alternate Names

Antagonist of beta-catenin Dapper homolog 3; arginine rich region 1; Arginine-rich region 1 protein; DACT3; Dapper 3; dapper homolog 3; dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis); MGC15476; RRR1