Human/Mouse DACT3/Dapper 3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse DACT3/Dapper 3 by Western Blot.
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Product Details
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Human/Mouse DACT3/Dapper 3 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse DACT3/Dapper 3
Accession # Q0PHV7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse DACT3/Dapper 3 antibody by Western Blot. View Larger

Detection of Human and Mouse DACT3/Dapper 3 by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse DACT3/Dapper 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for DACT3/Dapper 3 at approximately 45 kDa with human brain and 50 kDa with mouse brain (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DACT3/Dapper 3

Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins.  It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta -catenin signaling.  When present in colorectal tumor cells, it initiates apoptosis.  Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta -catenin entry into the nucleus.  Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610).  There is one potential splice variant that shows a 45 aa substitution for aa 413-610.  In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.      


Long Name
Dapper, Antagonist of beta-Catenin, Homolog 3
Entrez Gene IDs
147906 (Human); 629378 (Mouse); 499088 (Rat)
Alternate Names
Antagonist of beta-catenin Dapper homolog 3; arginine rich region 1; Arginine-rich region 1 protein; DACT3; Dapper 3; dapper homolog 3; dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis); MGC15476; RRR1

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Secondary Antibodies

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