Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody

R&D Systems | Catalog # FAB1981R

R&D Systems
Discontinued Product
FAB1981R has been discontinued. View all Frizzled-7 products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 151143
Loading...

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090

Specificity

Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Retinoic acid‑treated D3 mouse embryonic stem cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

References

  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Medina, A. et al. (2000) Mech. Dev. 92:227.
  5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  8. Vincan, E. et al. (2007) Oncogene 26:2340.

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Gene Symbol

FZD7

UniProt

Additional Frizzled-7 Products

Product Documents for Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews for Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody and earn rewards!

Have you used Human/Mouse Frizzled-7 Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies