Human/Mouse HIF-1 alpha/HIF1A Antibody

Catalog # Availability Size / Price Qty
HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue.
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Product Details
Citations (8)
Supplemental Products

Human/Mouse HIF-1 alpha/HIF1A Antibody Summary

Species Reactivity
Human, Mouse
Recognizes human and mouse HIF-1 alpha/HIF1A in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 241812
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HIF-1 alpha/HIF1A
Accession # Q16665.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
CoCl2‑treated MCF‑7 human breast cancer cell line, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HIF-1 alpha/HIF1A antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

HIF-1 alpha/HIF1A in Human Kidney Cancer Tissue. HIF-1 alpha/HIF1A was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse HIF-1 alpha/HIF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. microRNA-142 guards against autoimmunity by�controlling�Treg�cell homeostasis and function
    Authors: WL Wang, C Ouyang, NM Graham, Y Zhang, K Cassady, EY Reyes, M Xiong, AM Davis, K Tang, D Zeng, MP Boldin
    PloS Biology, 2022;20(2):e3001552.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tumor hypoxia represses &gamma&delta T cell-mediated antitumor immunity against brain tumors
    Authors: JH Park, HJ Kim, CW Kim, HC Kim, Y Jung, HS Lee, Y Lee, YS Ju, JE Oh, SH Park, JH Lee, SK Lee, HK Lee
    Nature Immunology, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells
    Authors: Y Sun, X Lin, B Liu, Y Zhang, W Li, S Zhang, F He, H Tian, X Zhu, X Liu, J Wu, J Cai, M Li
    Science Advances, 2021;7(52):eabj6877.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Celastrol mediates Th17 and Treg cell generation via metabolic signaling
    Authors: J Zhang, J Shan, X Chen, S Li, D Long, Y Li
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
    Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
    Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Loss of VHL in mesenchymal progenitors of the limb bud alters multiple steps of endochondral bone development.
    Authors: Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard-O'Riley R, Wilson T, Rankin E, Giaccia A, Schipani E
    Dev Biol, 2014;393(1):124-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
    Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S
    Cancer Res., 2007;67(12):5789-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
    Authors: Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J
    Int J Clin Exp Pathol, 0;7(2):575-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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