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Human/Mouse HSF4 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB5695V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5695V-100UG

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Human/Mouse HSF4 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse HSF4 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 504524
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human HSF4
Ser309-Pro463
Accession # BAA13433
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF4

HSF4 (heat shock factor 4) is a 53 kDa member of the HSF family of transcription factors. It is highly expressed in the lens where it maintains lens integrity and blocks FGF synthesis. Human HSF4 has two isoforms HSF4B and HSF4A. Human HSF4B is 493 amino acids in length. It contains one DNA binding region (aa 18‑122) and two hydrophobic repeat domains A/B and C (aa 130‑204 and 369‑390). HSF4B activates transcription, whereas, HSF4A represses transcription. HSF4A shows a 45 aa substitution for aa 246‑320. Over aa 309‑463, human HSF4B shares 73% aa sequence identity with mouse HSF4B.

Long Name
Heat Shock Transcription Factor 4
Entrez Gene IDs
3299 (Human); 26386 (Mouse)
Alternate Names
ATHSF4; AT-HSFB1; cataract, Marner; CTM; heat shock factor protein 4; heat shock transcription factor 4CTM; hHSF4; HSF 4; HSF4; HSFB1; HSTF 4

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