Detection of Human/Mouse HSF4 by Western Blot.
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, human LNCaP human prostate cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF4 Monoclonal Antibody (Catalog # MAB5695) followed by HRP-conjugated Anti‑Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
HSF4 (heat shock factor 4) is a 53 kDa member of the HSF family of transcription factors. It is highly expressed in the lens where it maintains lens integrity and blocks FGF synthesis. Human HSF4 has two isoforms HSF4B and HSF4A. Human HSF4B is 493 amino acids in length. It contains one DNA binding region (aa 18‑122) and two hydrophobic repeat domains A/B and C (aa 130‑204 and 369‑390). HSF4B activates transcription, whereas, HSF4A represses transcription. HSF4A shows a 45 aa substitution for aa 246‑320. Over aa 309‑463, human HSF4B shares 73% aa sequence identity with mouse HSF4B.
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