Human/Mouse HSF4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse HSF4 by Western Blot.
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Product Details
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Human/Mouse HSF4 Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse HSF4 in Western blots.
Monoclonal Mouse IgG1 Clone # 504524
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HSF4
Accession # BAA13433
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse HSF4 antibody by Western Blot. View Larger

Detection of Human/Mouse HSF4 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human LNCaP human prostate cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF4 Monoclonal Antibody (Catalog # MAB5695) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSF4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSF4

HSF4 (heat shock factor 4) is a 53 kDa member of the HSF family of transcription factors. It is highly expressed in the lens where it maintains lens integrity and blocks FGF synthesis. Human HSF4 has two isoforms HSF4B and HSF4A. Human HSF4B is 493 amino acids in length. It contains one DNA binding region (aa 18‑122) and two hydrophobic repeat domains A/B and C (aa 130‑204 and 369‑390). HSF4B activates transcription, whereas, HSF4A represses transcription. HSF4A shows a 45 aa substitution for aa 246‑320. Over aa 309‑463, human HSF4B shares 73% aa sequence identity with mouse HSF4B.

Long Name
Heat Shock Transcription Factor 4
Entrez Gene IDs
3299 (Human); 26386 (Mouse)
Alternate Names
ATHSF4; AT-HSFB1; cataract, Marner; CTM; heat shock factor protein 4; heat shock transcription factor 4CTM; hHSF4; HSF 4; HSF4; HSFB1; HSTF 4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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