Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 299612
View all Netrin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Netrin-4
Gly19-Val628
Accession # Q9JI33

Specificity

Detects human and mouse Netrin‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-G1a, rmNetrin-G2a, recombinant human Netrin-4, recombinant chicken (rch) Netrin-1, or rchNetrin‑2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse Netrin‑4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Netrin-4 (Catalog # 1254-N4)
Recombinant Mouse Netrin-4 (Catalog # 1132-N4)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-4

Netrins are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons.

Alternate Names

beta-Netrin, Netrin4, NTN4

Entrez Gene IDs

59277 (Human); 57764 (Mouse)

Gene Symbol

NTN4

UniProt

Q9JI33

Additional Netrin-4 Products

Product Documents for Human/Mouse Netrin‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Netrin‑4 Antibody

For research use only

Citations for Human/Mouse Netrin‑4 Antibody

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FAQs

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