Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody
Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody Summary
Met1-Asn265 (Met262Leu)
Accession # Q01860
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Oct‑3/4 in Mouse Embryonic Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 2 days were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oct-3/4
Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
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Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
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Scholer, H.R. et al. (1989) EMBO J. 8:2543.
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Rosner, M.H. et al. (1990) Nature 345:686.
- Niwa, H. et al. (2000) Nat. Genet. 24:372.
Product Datasheets
Citations for Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
BMC Cell Biol., 2017;18(1):21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemoresistant lung cancer stem cells display high DNA repair capability to remove cisplatin-DNA damage
Authors: Wai-Kin Yu
Br. J. Pharmacol, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
Biotechnol. Bioeng., 2009;102(6):1636-44.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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