Human/Mouse/Primate Dopamine beta-Hydroxylase C-Terminus Antibody

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Detection of Dopamine  beta -Hydroxylase (C-Terminus) by Western blot.
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Human/Mouse/Primate Dopamine beta-Hydroxylase C-Terminus Antibody Summary

Species Reactivity
Human, Mouse, Primate
Human, monkey, bovine, and rabbit ~75 kDa Dopamine beta -hydroxylase (D beta H)
Polyclonal Sheep IgG
Antigen Affinity-purified
Dopamine beta-Hydroxylase
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, and 0.09% sodium azide.


Recommended Concentration
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Dopamine  beta -Hydroxylase (C-Terminus) antibody by Western Blot. View Larger

Detection of Dopamine beta -Hydroxylase (C-Terminus) by Western blot. Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Dopamine beta-Hydroxylase

Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu+-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl- concentrations.

  1. Kreek, M.J. et al. (2005) Pharmacol. Rev. 57:1.
  2. Stewart, L. and J.P. Klinman (1999) FEBS Lett. 454:229.
  3. Houhou, L. et al. (1995) J. Biol. Chem. 270:12601.
  4. Timmers, H. et al. (2004) Ann. N.Y. Acad. Sci. 1018:520.
  5. Lamouroux, A. et al. (1987) EMBO J. 6:3931.
Entrez Gene IDs
1621 (Human); 13166 (Mouse); 25699 (Rat)
Alternate Names
DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC

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Product Specific Notices

* This product contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.


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