Human/Mouse/Primate Dopamine beta -Hydroxylase C-Terminus Antibody

  
  • Species Reactivity
    Human, Mouse, Primate
  • Specificity
    Human, monkey, bovine, and rabbit ~75 kDa Dopamine beta -hydroxylase (D beta H)
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Dopamine beta-Hydroxylase
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, and 0.09% sodium azide.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Dopamine beta -Hydroxylase
(C-Terminus) by Western blot.
Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Dopamine beta-Hydroxylase

Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu+-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl- concentrations.

  • References:
    1. Kreek, M.J. et al. (2005) Pharmacol. Rev. 57:1.
    2. Stewart, L. and J.P. Klinman (1999) FEBS Lett. 454:229.
    3. Houhou, L. et al. (1995) J. Biol. Chem. 270:12601.
    4. Timmers, H. et al. (2004) Ann. N.Y. Acad. Sci. 1018:520.
    5. Lamouroux, A. et al. (1987) EMBO J. 6:3931.
  • Entrez Gene IDs:
    1621 (Human); 13166 (Mouse); 25699 (Rat)
  • Alternate Names:
    DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC 1.14.17.1
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