Human/Mouse/Primate Dopamine beta-Hydroxylase C-Terminus Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Dopamine beta -Hydroxylase (C-Terminus) by Western blot. Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein.
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Background: Dopamine beta-Hydroxylase
Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu+-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl- concentrations.
- Kreek, M.J. et al. (2005) Pharmacol. Rev. 57:1.
- Stewart, L. and J.P. Klinman (1999) FEBS Lett. 454:229.
- Houhou, L. et al. (1995) J. Biol. Chem. 270:12601.
- Timmers, H. et al. (2004) Ann. N.Y. Acad. Sci. 1018:520.
- Lamouroux, A. et al. (1987) EMBO J. 6:3931.
Product Specific Notices* This product contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.
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