Human/Mouse Rab7a Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Rab7a

Rab7a (Ras-related protein Rab 7A; also RAB7) is a 22-24 kDa member of the Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as skeletal muscle, neurons, osteoclasts and macrophages. Rab7a plays a key role in the formation of phagolysosomes. In particular, Rab7a-GTP becomes associated with early phagosomes. The GTP form of Rab7a first recruits RILP, a molecule that interacts with ORPL1 and promotes the formation of a dynactin:dynein complex. This complex now initiates the movement of the phagosome along microtubules, where it fuses with lysosomes and endosomes at select points, creating phagolysosomes. Human Rab7a is 207 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C‑terminal CXC prenylation sequence (aa 205-207). There is one utilized phosphorylation site at Tyr183. Full-length human and mouse Rab7a have identical aa sequences. Notably, however, point mutations with single aa changes are noted in both species. By contrast, human Rab7a and Rab7b share less than 50% aa sequence identity, each representing the product of distinct genes.

Product Datasheets

Related Research Areas

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