Human/Mouse/Rat BNIP3L Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4030N Datasheet / COA / SDS

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AF4030N-100UG

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Human/Mouse/Rat BNIP3L Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BNIP3L
Ser2-Glu184
Accession # O60238

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BNIP3L

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219 amino acid (aa), 24 kDa (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Entrez Gene IDs

665 (Human); 12177 (Mouse); 140923 (Rat)

Alternate Names

Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19kDa interacting protein 3-like; BNIP3a; BNIP3H; BNIP3L; Nip3L; NIP3-like protein X; Nix; NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like