Human/Mouse/Rat BNIP3L Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat BNIP3L by Western Blot.
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Product Details
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Human/Mouse/Rat BNIP3L Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human BNIP3L
Accession # O60238
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat BNIP3L antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat BNIP3L by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3L at approximately 35kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry BNIP3L antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

BNIP3L in HeLa Human Cell Line. BNIP3L was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNIP3L

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219 amino acid (aa), 24 kDa (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.

Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Entrez Gene IDs
665 (Human); 12177 (Mouse); 140923 (Rat)
Alternate Names
Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19kDa interacting protein 3-like; BNIP3a; BNIP3H; BNIP3L; Nip3L; NIP3-like protein X; Nix; NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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