Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BNIP3L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BNIP3L
Ser2-Glu184
Accession # O60238

Specificity

Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat BNIP3L Antibody

Detection of Human/Mouse/Rat BNIP3L antibody by Western Blot.

Detection of Human/Mouse/Rat BNIP3L by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3L at approximately 35kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
BNIP3L antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

BNIP3L in HeLa Human Cell Line.

BNIP3L was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat BNIP3L Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 4 using AF4030 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BNIP3L

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219 amino acid (aa), 24 kDa (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

BNIP3a, Nip3L, Nix

Entrez Gene IDs

665 (Human); 12177 (Mouse); 140923 (Rat)

Gene Symbol

BNIP3L

UniProt

O60238

Additional BNIP3L Products

Product Documents for Human/Mouse/Rat BNIP3L Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat BNIP3L Antibody

For research use only

Related Research Areas

Autophagy
Bcl-2 Proteins and Regulators

Citations for Human/Mouse/Rat BNIP3L Antibody

Customer Reviews for Human/Mouse/Rat BNIP3L Antibody (1)

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  • Human/Mouse/Rat BNIP3L Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone Marrrow Derived Macrophages
    Species: Mouse
    Verified Customer | Posted 10/15/2020
    Western blot of BMDM cell lysates incubated with 1:800 dilution of the antibody and detected with anti-goat HRP.
    Human/Mouse/Rat BNIP3L Antibody AF4030

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Protocols

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