FK506 binding protein, 25 kDa molecular weight (FKBP25), also called FKBP3, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant Rapamycin is a potent inhibitor of FKBP25. FKBP25 is localized in the nucleus, where it alters the activity of histone deacetylases and transcription factors. It is found in many tissues, including brain, thymus, and spleen.
FKBP25 Antibody (423325)
R&D Systems | Catalog # MAB3955
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 423325
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Product Specifications
Immunogen
E. coli-derived recombinant human FKBP25
Ala2-Asp224
Accession # Q00688
Ala2-Asp224
Accession # Q00688
Specificity
Detects endogenous human, mouse, and rat FKBP25 in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP13, FKBP38, FKBP51, or FKBP52.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for FKBP25 Antibody (423325)
Detection of Human FKBP25 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP25 Monoclonal Antibody (Catalog # MAB3955) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP25 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for FKBP25 Antibody (423325)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and CHP-100 human neuroblastoma cell line
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and CHP-100 human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FKBP25
Long Name
25 kDa FK506 Binding Protein
Alternate Names
FKBP3
Gene Symbol
FKBP3
UniProt
Additional FKBP25 Products
Product Documents for FKBP25 Antibody (423325)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for FKBP25 Antibody (423325)
For research use only
Related Research Areas
Citations for FKBP25 Antibody (423325)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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